Peptide Reconstitution Calculator
Concentration math for the bench: enter a lyophilized mass and diluent volume to get mg/mL and per-aliquot figures — or work backwards from a target concentration.
Figures are laboratory solution math only — not preparation, handling, or use instructions for any application. Research compounds are not for human or veterinary use.
How the math works
Reconstitution concentration is a single division: concentration = peptide mass ÷ diluent volume. A 10 mg vial reconstituted with 2 mL of bacteriostatic water gives 5 mg/mL; the same vial with 5 mL gives 2 mg/mL. More diluent means a more dilute solution of the same total mass — nothing is gained or lost, only spread across more volume. Working backwards is the same equation rearranged: the volume required for a target concentration is mass ÷ target concentration.
Diluent choice affects working life, not concentration: bacteriostatic water's preservative supports repeated draws over a multi-week refrigerated window, which is why it is the standard for laboratory reconstitution. Our guide on bacteriostatic vs. sterile water covers the difference, and every TrueBond compound ships with its lot's published COA.
Common questions
How is reconstitution concentration calculated?
Concentration equals peptide mass divided by diluent volume. Dissolving a 10 mg lyophilized peptide in 2 mL of bacteriostatic water yields 10 ÷ 2 = 5 mg/mL (5,000 µg/mL). The calculator also expresses the peptide mass contained in common aliquot volumes of that solution.
Does the powder itself add volume?
At research-vial quantities the lyophilized cake's contribution to final solution volume is negligible for bench math — a few milligrams of peptide displace almost nothing. Treating final volume as equal to diluent volume is the standard simplification.
What diluent do these figures assume?
The math is diluent-agnostic, but multi-draw laboratory work conventionally uses bacteriostatic water (0.9% benzyl alcohol), which supports a multi-week refrigerated working window that plain sterile water does not. See our guide comparing the two.
How should reconstituted solutions be stored?
General practice for reconstituted research peptides is refrigeration at 2–8°C, protection from light, and use within roughly 30 days — with the compound's own data sheet governing wherever it specifies otherwise.